Chapter title |
A Protocol for Studying Embryonic Mammary Progenitor Cells During Mouse Mammary Primordial Development in Explant Culture
|
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Chapter number | 2 |
Book title |
Mammary Stem Cells
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2519-3_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2518-6, 978-1-4939-2519-3
|
Authors |
Naoko Kogata, Beatrice A. Howard |
Abstract |
Embryonic explant culture is a powerful technique to observe tissue morphogenesis ex vivo, and is particularly useful for monitoring embryonic mammary gland development. It has been established that mammary cell lineage specification occurs during embryogenesis, although much remains to be elucidated with respect to how this occurs. During mammary specification, mammary progenitor cells are formed. Embryonic mammary development can proceed and be monitored in embryonic explant culture. Studies using explant culture will greatly enhance our understanding of the cellular mechanisms that regulate embryonic mammary primordial development and mammary progenitor cell specification. We present a protocol for culturing explants from mid-gestation mouse embryos so that morphogenetic processes and mammary epithelial progenitor cells can be studied during embryonic mammary development ex vivo. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 20% |
Student > Postgraduate | 1 | 20% |
Student > Master | 1 | 20% |
Unknown | 2 | 40% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 40% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 20% |
Unknown | 2 | 40% |