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Ribosome Display and Related Technologies

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Cover of 'Ribosome Display and Related Technologies'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Ribosome Display: A Perspective
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    Chapter 2 Preparation and Testing of E. coli S30 In Vitro Transcription Translation Extracts
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    Chapter 3 Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate
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    Chapter 4 Stabilized Ribosome Display for In Vitro Selection
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    Chapter 5 Eukaryotic Ribosome Display with In Situ DNA Recovery
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    Chapter 6 mRNA display using covalent coupling of mRNA to translated proteins.
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    Chapter 7 SNAP Display: In Vitro Protein Evolution in Microdroplets.
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    Chapter 8 cDNA Display: Rapid Stabilization of mRNA Display
  10. Altmetric Badge
    Chapter 9 Ribosome Display and Related Technologies
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    Chapter 10 Ribosome Display and Related Technologies
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    Chapter 11 Evolution of Protein Stability Using Ribosome Display
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    Chapter 12 Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries
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    Chapter 13 Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display
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    Chapter 14 Peptide Screening Using PURE Ribosome Display
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    Chapter 15 Rapid Selection of High-Affinity Binders Using Ribosome Display
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    Chapter 16 mRNA display-based selections using synthetic Peptide and natural protein libraries.
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    Chapter 17 Identification of Candidate Vaccine Genes Using Ribosome Display
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    Chapter 18 Ribosome Display for the Selection of Sac7d Scaffolds
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    Chapter 19 Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers
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    Chapter 20 Update on Pure Translation Display with Unnatural Amino Acid Incorporation
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    Chapter 21 In Vitro Selection of Unnatural Cyclic Peptide Libraries via mRNA Display.
  23. Altmetric Badge
    Chapter 22 Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display.
  24. Altmetric Badge
    Chapter 23 Ribosome Display and Related Technologies
Attention for Chapter 21: In Vitro Selection of Unnatural Cyclic Peptide Libraries via mRNA Display.
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Chapter title
In Vitro Selection of Unnatural Cyclic Peptide Libraries via mRNA Display.
Chapter number 21
Book title
Ribosome Display and Related Technologies
Published in
Methods in molecular biology, November 2011
DOI 10.1007/978-1-61779-379-0_21
Pubmed ID
Book ISBNs
978-1-61779-378-3, 978-1-61779-379-0
Authors

Ma Z, Hartman MC, Zhong Ma, Matthew C. T. Hartman

Abstract

The ribosomal synthesis of drug-like peptides containing unnatural amino acids is possible due to the broad substrate specificity of the ribosome. In this protocol, a reconstituted Escherichia coli ribosomal translation system (PURE) is adapted to incorporate unnatural amino acids into mRNA-displayed peptide libraries, which are used in in vitro selection.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 38%
Researcher 4 19%
Student > Doctoral Student 2 10%
Student > Bachelor 1 5%
Other 1 5%
Other 0 0%
Unknown 5 24%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 33%
Chemistry 5 24%
Biochemistry, Genetics and Molecular Biology 2 10%
Medicine and Dentistry 1 5%
Engineering 1 5%
Other 0 0%
Unknown 5 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 March 2012.
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#18,305,470
of 22,663,969 outputs
Outputs from Methods in molecular biology
#7,813
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Outputs of similar age
#195,097
of 238,870 outputs
Outputs of similar age from Methods in molecular biology
#306
of 447 outputs
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