Chapter title |
Ribosome Display and Related Technologies
|
---|---|
Chapter number | 23 |
Book title |
Ribosome Display and Related Technologies
|
Published in |
Methods in molecular biology, January 2012
|
DOI | 10.1007/978-1-61779-379-0_23 |
Pubmed ID | |
Book ISBNs |
978-1-61779-378-3, 978-1-61779-379-0
|
Authors |
Simon E. Hufton |
Abstract |
The pursuit of more potent, safe, and cost-effective drugs has placed a greater emphasis on antibody optimisation within the drug discovery process. Technologies to rapidly improve antibody drug performance, such as phage display, ribosome display, and yeast display, are playing a key role in this effort. Among these ribosome display is a particularly powerful technology and has recently been applied to the affinity optimisation of a humanised anti-receptor for advanced glycation end products (anti-RAGE) antibody (Finlay et al., J Mol Biol 388:541-558, 2009). By using a combination of error-prone PCR with ribosome display each amino acid position within this humanised antibody was scanned for both its functional importance and its capacity to increase affinity resulting in both affinity-matured antibody variants and a functional map of the antibody paratope. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 25% |
Unspecified | 1 | 13% |
Other | 1 | 13% |
Student > Bachelor | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Other | 2 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 50% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 13% |
Unspecified | 1 | 13% |
Immunology and Microbiology | 1 | 13% |
Engineering | 1 | 13% |
Other | 0 | 0% |