Chapter title |
Real-Time In Vivo Monitoring of Reactive Oxygen Species in Guard Cells
|
---|---|
Chapter number | 34 |
Book title |
Polyamines
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7398-9_34 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7397-2, 978-1-4939-7398-9
|
Authors |
Ky Young Park, Kalliopi A. Roubelakis-Angelakis |
Abstract |
The intra-/intercellular homeostasis of reactive oxygen species (ROS), and especially of superoxides (O2(.-)) and hydrogen peroxide (O2(.-)) participate in signalling cascades which dictate developmental processes and reactions to biotic/abiotic stresses. Polyamine oxidases terminally oxidize/back convert polyamines generating H2O2. Recently, an NADPH-oxidase/Polyamine oxidase feedback loop was identified to control oxidative burst under salinity. Thus, the real-time localization/monitoring of ROS in specific cells, such as the guard cells, can be of great interest. Here we present a detailed description of the real-time in vivo monitoring of ROS in the guard cells using H2O2(-) and O2(.-) specific fluorescing probes, which can be used for studying ROS accumulation generated from any source, including the amine oxidases-dependent pathway, during development and stress. |
Mendeley readers
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 13% |
Professor > Associate Professor | 1 | 13% |
Researcher | 1 | 13% |
Unknown | 3 | 38% |
Readers by discipline | Count | As % |
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Unknown | 2 | 25% |