Chapter title |
Identification of Branched-Chain Polyamines in Hyperthermophiles
|
---|---|
Chapter number | 8 |
Book title |
Polyamines
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7398-9_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7397-2, 978-1-4939-7398-9
|
Authors |
Ryota Hidese, Wakao Fukuda, Masaru Niitsu, Shinsuke Fujiwara, Hidese, Ryota, Fukuda, Wakao, Niitsu, Masaru, Fujiwara, Shinsuke |
Abstract |
Thermophiles are organisms that grow optimally at temperatures higher than 55 °C. They contain two types of unusual longer/branched-chain polyamines in addition to common polyamines such as spermidine and putrescine. These unusual polyamines contribute to the survival of hyperthermophiles at high temperatures. Recently, the novel aminopropyltransferase BpsA was found to be responsible for the biosynthesis of branched-chain polyamines in the hyperthermophilic archaeon Thermococcus kodakarensis, which contains N (4)-bis(aminopropyl)spermidine as the major polyamine. This compound is synthesized by the sequential addition of decarboxylated S-adenosylmethionine (dcSAM) aminopropyl groups to spermidine via the bifunctional catalytic action of BpsA. In this chapter, methods for the extraction and identification of branched-chain polyamines are presented, along with methods for the production and characterization of recombinant T. kodakarensis BpsA as a model aminopropyltransferase. |
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