Chapter title |
Techniques Used for Functional Characterization of Polyamine Transporters
|
---|---|
Chapter number | 21 |
Book title |
Polyamines
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7398-9_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7397-2, 978-1-4939-7398-9
|
Authors |
Claudio A. Pereira, Melisa Sayé, Chantal Reigada, Mariana R. Miranda |
Abstract |
Transport systems are key processes in every living organism: they allow the entry of all essential nutrients into the cell and its compartments and regulate the intracellular concentrations of metabolites. The transport of cell nutrients represents the first step of many metabolic routes and may also regulate such processes. They are also responsible for reaching the effective intracellular concentration of therapeutic drugs and some mechanisms of resistance and tolerance also depend on them. However, the common techniques used to evaluate the metabolites transport in different cells types are not easy to carry out and require extensive training. In this chapter, we report detailed protocols and tips about the expression of transporters, different activity assays and transporter kinetics determination. |
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