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Chapter title |
The EPR Method for Detecting Nitric Oxide in Plant Senescence
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Chapter number | 10 |
Book title |
Plant Senescence
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7672-0_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7670-6, 978-1-4939-7672-0
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Authors |
Aizhen Sun |
Abstract |
Nitric oxide (NO) is gaining increasing attention as a central molecule with diverse signaling functions. It has been shown that NO acts as a negative regulator of leaf senescence. In this chapter, we describe a highly selective method, electron paramagnetic resonance ([EPR], also known as electron spin resonance [ESR]), for NO determination in leaf senescence. An iron complex of ferrous and mononitrosyl dithiocarbamate (Fe2+(DETC)2) is used as a chelating agent for NO. Using ethyl acetate as extracting solvent, the NOFe2+(DETC)2 complex is extracted and determined by EPR spectrometer. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 1 | 25% |
Student > Ph. D. Student | 1 | 25% |
Researcher | 1 | 25% |
Student > Master | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 75% |
Biochemistry, Genetics and Molecular Biology | 1 | 25% |