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Mendeley readers
Chapter title |
Isolation of Chloroplasts for In Organelle Protein Degradation Assay
|
---|---|
Chapter number | 24 |
Book title |
Plant Senescence
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7672-0_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7670-6, 978-1-4939-7672-0
|
Authors |
Scott W. Vande Wetering, Judy A. Brusslan |
Abstract |
A method for isolating intact chloroplasts from mature and senescent Arabidopsis thaliana leaves is described that utilizes two subsequent Percoll gradients. The isolated chloroplasts can be incubated in the dark to track in organelle protein degradation. To remove chloroplasts that lysed during the incubation period, a second Percoll gradient is performed prior to SDS-PAGE and immunoblot analysis. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 2 | 67% |
Student > Doctoral Student | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Agricultural and Biological Sciences | 1 | 33% |
Chemistry | 1 | 33% |