Chapter title |
Two-electrode voltage clamp.
|
---|---|
Chapter number | 6 |
Book title |
Ion Channels
|
Published in |
Methods in molecular biology, March 2013
|
DOI | 10.1007/978-1-62703-351-0_6 |
Pubmed ID | |
Book ISBNs |
978-1-62703-350-3, 978-1-62703-351-0
|
Authors |
Guan B, Chen X, Zhang H, Bingcai Guan, Xingjuan Chen, Hailin Zhang, Guan, Bingcai, Chen, Xingjuan, Zhang, Hailin |
Abstract |
Two-electrode voltage clamp (TEVC) is a conventional electrophysiological technique used to artificially control the membrane potential (V m) of large cells to study the properties of electrogenic membrane proteins, especially ion channels. It makes use of two intracellular electrodes-a voltage electrode as V m sensor and a current electrode for current injection to adjust the V m, thus setting the membrane potential at desired values and recording the membrane current to analyze ion channel activities. Here we describe the use of TEVC in combination with exogenous mRNA expression in Xenopus oocytes for ion channel recording. |
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