Chapter title |
Correlation of Glioma Proliferation and Hypoxia by Luciferase, Magnetic Resonance, and Positron Emission Tomography Imaging
|
---|---|
Chapter number | 26 |
Book title |
Hypoxia
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7665-2_26 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7664-5, 978-1-4939-7665-2
|
Authors |
Michael Karsy, David L. Gillespie, Kevin P. Horn, Lance D. Burrell, Jeffery T. Yap, Randy L. Jensen |
Abstract |
Gliomas are the most common type of primary, malignant brain tumor and significantly impact patients, who have a median survival of ~1 year depending on mutational background. Novel imaging modalities such as luciferase bioluminescence, micro-magnetic resonance imaging (micro-MRI), micro-computerized tomography (micro-CT), and micro-positron emission tomography (micro-PET) have expanded the portfolio of tools available to study this disease. Hypoxia, a key oncogenic driver of glioma and mechanism of resistance, can be studied in vivo by the concomitant use of noninvasive MRI and PET imaging. We present a protocol involving stereotactic injection of syngenic F98 luciferase-expressing glioma cells generated by our laboratory into Fischer 344 rat brains and imaging using luciferase. In addition, 18-F-fludeoxyglucose, 18F-fluoromisonidazole, and 18F-fluorothymidine PET imaging are compared with quantified luciferase flux. These tools can potentially be used for assessing tumor growth characteristics, hypoxia, mutational effects, and treatment effects. |
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