You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
Acquisition of Temporal HIF Transcriptional Activity Using a Secreted Luciferase Assay
|
---|---|
Chapter number | 4 |
Book title |
Hypoxia
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7665-2_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7664-5, 978-1-4939-7665-2
|
Authors |
Miguel A. S. Cavadas, Cormac T. Taylor, Alex Cheong, Cavadas, Miguel A. S., Taylor, Cormac T., Cheong, Alex |
Abstract |
Here we describe a simple method based on secreted luciferase driven by a hypoxia-inducible factor (HIF) response element (HRE) that allows the acquisition of dynamic and high-throughput data on HIF transcriptional activity during hypoxia and pharmacological activation of HIF. The sensitivity of the assay allows for the secreted luciferase to be consecutively sampled (as little as 1% of the total supernatant) over an extended time period, thus allowing the acquisition of time-resolved HIF transcriptional activity. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 50% |
Student > Ph. D. Student | 1 | 25% |
Student > Postgraduate | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 75% |
Economics, Econometrics and Finance | 1 | 25% |