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Mendeley readers
| Chapter title |
5′-Bromouridine IP Chase (BRIC)-Seq to Determine RNA Half-Lives
|
|---|---|
| Chapter number | 1 |
| Book title |
mRNA Decay
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7540-2_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7539-6, 978-1-4939-7540-2
|
| Authors |
Toshimichi Yamada, Naoto Imamachi, Rena Onoguchi-Mizutani, Katsutoshi Imamura, Yutaka Suzuki, Nobuyoshi Akimitsu |
| Abstract |
Analysis of RNA stability at genome-wide level is an advanced method in RNA biology that examines the half-life of each transcript. In particular, a pulse-labeling method using uridine analogs enables the determination of half-life of each transcript under physiologically undisturbed conditions. The technique involves pulse labeling of endogenous RNAs in mammalian cells with 5'-bromouridine (BrU), followed by measuring the chronological decrease of BrU-labeled RNAs using deep sequencing (BRIC-seq). Here, we describe a detailed protocol and technical tips for BRIC-seq. |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 19% |
| Student > Bachelor | 2 | 13% |
| Professor | 2 | 13% |
| Student > Doctoral Student | 2 | 13% |
| Student > Master | 2 | 13% |
| Other | 3 | 19% |
| Unknown | 2 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 50% |
| Medicine and Dentistry | 3 | 19% |
| Agricultural and Biological Sciences | 2 | 13% |
| Immunology and Microbiology | 1 | 6% |
| Unknown | 2 | 13% |