Chapter title |
PAR-CLIP for Discovering Target Sites of RNA-Binding Proteins
|
---|---|
Chapter number | 5 |
Book title |
Methods in Molecular Biology
|
Published in |
Methods in molecular biology, December 2017
|
DOI | 10.1007/978-1-4939-7540-2_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7539-6, 978-1-4939-7540-2
|
Authors |
Garzia, Aitor, Morozov, Pavel, Sajek, Marcin, Meyer, Cindy, Tuschl, Thomas, Aitor Garzia, Pavel Morozov, Marcin Sajek, Cindy Meyer, Thomas Tuschl |
Abstract |
RNA-binding proteins (RBPs) establish posttranscriptional gene regulation (PTGR) by coordinating the maturation, editing, transport, stability, and translation of cellular RNAs. A variety of experimental approaches have been developed to characterize the RNAs associated with RBPs in vitro as well as in vivo. Our laboratory developed Photoactivatable-Ribonucleoside-Enhanced Cross-Linking and Immunoprecipitation (PAR-CLIP), which in combination with next-generation sequencing enables the identification of RNA targets of RBPs at a nucleotide-level resolution. Here we present an updated and condensed step-by-step PAR-CLIP protocol followed by the description of our RNA-seq data analysis pipeline. |
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Demographic breakdown
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Researcher | 6 | 22% |
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Other | 3 | 11% |
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