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Chromatin Immunoprecipitation

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Cover of 'Chromatin Immunoprecipitation'

Table of Contents

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    Book Overview
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    Chapter 1 ChIP and ChIP-Related Techniques: Expanding the Fields of Application and Improving ChIP Performance
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    Chapter 2 Considerations on Experimental Design and Data Analysis of Chromatin Immunoprecipitation Experiments
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    Chapter 3 How to Combine ChIP with qPCR
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    Chapter 4 Analysis of Protein–DNA Interaction by Chromatin Immunoprecipitation and DNA Tiling Microarray (ChIP-on-chip)
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    Chapter 5 Chromatin Immunoprecipitation from Mouse Embryonic Tissue or Adherent Cells in Culture, Followed by Next-Generation Sequencing
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    Chapter 6 Chromatin RNA Immunoprecipitation (ChRIP)
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    Chapter 7 DNA Accessibility by MNase Digestions
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    Chapter 8 Characterization of the Nucleosome Landscape by Micrococcal Nuclease-Sequencing (MNase-seq)
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    Chapter 9 ChIP-re-ChIP: Co-occupancy Analysis by Sequential Chromatin Immunoprecipitation
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    Chapter 10 Sm-ChIPi: Single-Molecule Chromatin Immunoprecipitation Imaging
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    Chapter 11 Chromatin Immunoprecipitation of Skeletal Muscle Tissue
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    Chapter 12 Chromatin Immunoprecipitation Assay in the Hyperthermoacidophilic Crenarchaeon, Sulfolobus acidocaldarius
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    Chapter 13 Using Intra-ChIP to Measure Protein–DNA Interactions in Intracellular Pathogens
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    Chapter 14 Native Chromatin Immunoprecipitation-Sequencing (ChIP-Seq) from Low Cell Numbers
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    Chapter 15 MOBE-ChIP: Probing Cell Type-Specific Binding Through Large-Scale Chromatin Immunoprecipitation
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    Chapter 16 Multiplexed ChIP-Seq Using Direct Nucleosome Barcoding: A Tool for High-Throughput Chromatin Analysis
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    Chapter 17 Analysis of ChIP-seq Data in R/Bioconductor
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    Chapter 18 Spike-In Normalization of ChIP Data Using DNA–DIG–Antibody Complex
Attention for Chapter 4: Analysis of Protein–DNA Interaction by Chromatin Immunoprecipitation and DNA Tiling Microarray (ChIP-on-chip)
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Chapter title
Analysis of Protein–DNA Interaction by Chromatin Immunoprecipitation and DNA Tiling Microarray (ChIP-on-chip)
Chapter number 4
Book title
Chromatin Immunoprecipitation
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7380-4_4
Pubmed ID
Book ISBNs
978-1-4939-7379-8, 978-1-4939-7380-4
Authors

Hui Gao, Chunyan Zhao

Abstract

Chromatin immunoprecipitation (ChIP) has become the most effective and widely used tool to study the interactions between specific proteins or modified forms of proteins and a genomic DNA region. Combined with genome-wide profiling technologies, such as microarray hybridization (ChIP-on-chip) or massively parallel sequencing (ChIP-seq), ChIP could provide a genome-wide mapping of in vivo protein-DNA interactions in various organisms. Here, we describe a protocol of ChIP-on-chip that uses tiling microarray to obtain a genome-wide profiling of ChIPed DNA.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 33%
Student > Bachelor 1 33%
Unknown 1 33%
Readers by discipline Count As %
Unspecified 1 33%
Biochemistry, Genetics and Molecular Biology 1 33%
Unknown 1 33%