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Chapter title |
Analysis of Protein–DNA Interaction by Chromatin Immunoprecipitation and DNA Tiling Microarray (ChIP-on-chip)
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Chapter number | 4 |
Book title |
Chromatin Immunoprecipitation
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7380-4_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7379-8, 978-1-4939-7380-4
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Authors |
Hui Gao, Chunyan Zhao |
Abstract |
Chromatin immunoprecipitation (ChIP) has become the most effective and widely used tool to study the interactions between specific proteins or modified forms of proteins and a genomic DNA region. Combined with genome-wide profiling technologies, such as microarray hybridization (ChIP-on-chip) or massively parallel sequencing (ChIP-seq), ChIP could provide a genome-wide mapping of in vivo protein-DNA interactions in various organisms. Here, we describe a protocol of ChIP-on-chip that uses tiling microarray to obtain a genome-wide profiling of ChIPed DNA. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 1 | 33% |
Student > Bachelor | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 33% |
Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Unknown | 1 | 33% |