Chapter title |
Multiplexed ChIP-Seq Using Direct Nucleosome Barcoding: A Tool for High-Throughput Chromatin Analysis
|
---|---|
Chapter number | 16 |
Book title |
Chromatin Immunoprecipitation
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7380-4_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7379-8, 978-1-4939-7380-4
|
Authors |
Christophe D. Chabbert, Sophie H. Adjalley, Lars M. Steinmetz, Vicent Pelechano |
Abstract |
Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) or microarray hybridization (ChIP-on-chip) are standard methods for the study of transcription factor binding sites and histone chemical modifications. However, these approaches only allow profiling of a single factor or protein modification at a time.In this chapter, we present Bar-ChIP, a higher throughput version of ChIP-Seq that relies on the direct ligation of molecular barcodes to chromatin fragments. Bar-ChIP enables the concurrent profiling of multiple DNA-protein interactions and is therefore amenable to experimental scale-up, without the need for any robotic instrumentation. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 20% |
Germany | 1 | 20% |
France | 1 | 20% |
Unknown | 2 | 40% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 3 | 60% |
Members of the public | 2 | 40% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 21% |
Professor | 2 | 14% |
Student > Ph. D. Student | 2 | 14% |
Student > Postgraduate | 2 | 14% |
Student > Master | 2 | 14% |
Other | 1 | 7% |
Unknown | 2 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 64% |
Agricultural and Biological Sciences | 4 | 29% |
Unknown | 1 | 7% |