Chapter title |
Cell Death and Survival Assays
|
---|---|
Chapter number | 9 |
Book title |
Chaperones
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7477-1_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7476-4, 978-1-4939-7477-1
|
Authors |
Alexander E. Kabakov, Vladimir L. Gabai |
Abstract |
Heat shock proteins are well-known protectors from cell death. Cell death (in particular, apoptosis and necrosis) is accompanied by certain hallmarks manifested as specific alterations in cellular membranes, cytoplasm, nucleus, and mitochondria. Some of those hallmarks are easily detectable in situ and, therefore, they can be applied for the assessment of dying or dead cells. In turn, there are also signs of viable cells that include such features as normal functioning of their membranes and organelles, ability to proliferate, etc. This chapter describes several convenient methods for quantification of dead (apoptotic and necrotic) cells as well as methods for assessment of viable cells. We describe in detail methods of annexin V/propidium iodide (PI) staining, TUNEL assay, Hoechst/PI staining, caspase activation, MTS tetrazolium, lactate dehydrogenase (LDH) release, colony formation, and senescence assays, with the principles, advantages, and drawbacks of each technique. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 155 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 28 | 18% |
Student > Ph. D. Student | 20 | 13% |
Student > Master | 18 | 12% |
Student > Doctoral Student | 9 | 6% |
Student > Postgraduate | 7 | 5% |
Other | 15 | 10% |
Unknown | 58 | 37% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 35 | 23% |
Medicine and Dentistry | 11 | 7% |
Agricultural and Biological Sciences | 9 | 6% |
Pharmacology, Toxicology and Pharmaceutical Science | 9 | 6% |
Immunology and Microbiology | 5 | 3% |
Other | 19 | 12% |
Unknown | 67 | 43% |