Chapter title |
A Transcriptomic Approach to Identify Novel Drug Efflux Pumps in Bacteria
|
---|---|
Chapter number | 12 |
Book title |
Bacterial Multidrug Exporters
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7454-2_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7452-8, 978-1-4939-7454-2
|
Authors |
Liping Li, Sasha G. Tetu, Ian T. Paulsen, Karl A. Hassan |
Abstract |
The core genomes of most bacterial species include a large number of genes encoding putative efflux pumps. The functional roles of most of these pumps are unknown, however, they are often under tight regulatory control and expressed in response to their substrates. Therefore, one way to identify pumps that function in antimicrobial resistance is to examine the transcriptional responses of efflux pump genes to antimicrobial shock. By conducting complete transcriptomic experiments following antimicrobial shock treatments, it may be possible to identify novel drug efflux pumps encoded in bacterial genomes. In this chapter we describe a complete workflow for conducting transcriptomic analyses by RNA sequencing, to determine transcriptional changes in bacteria responding to antimicrobials. |
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