You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
| Chapter title |
Purification of AcrAB-TolC Multidrug Efflux Pump for Cryo-EM Analysis
|
|---|---|
| Chapter number | 5 |
| Book title |
Bacterial Multidrug Exporters
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7454-2_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7452-8, 978-1-4939-7454-2
|
| Authors |
Dijun Du, Zhao Wang, Wah Chiu, Ben F. Luisi, Du, Dijun, Wang, Zhao, Chiu, Wah, Luisi, Ben F. |
| Abstract |
The cell envelope of Gram-negative bacteria comprises an outer membrane, a cytoplasmic inner membrane, and an interstitial space. The tripartite multidrug transporter AcrAB-TolC, which uses proton electrochemical gradients to vectorially drive the efflux of drugs from the cell, spans this envelope. We describe here details of the methods used to prepare the recombinant tripartite assembly for high-resolution structure determination by cryo-EM. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 30% |
| Student > Ph. D. Student | 2 | 20% |
| Student > Bachelor | 1 | 10% |
| Professor > Associate Professor | 1 | 10% |
| Unknown | 3 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 30% |
| Immunology and Microbiology | 1 | 10% |
| Chemistry | 1 | 10% |
| Medicine and Dentistry | 1 | 10% |
| Unknown | 4 | 40% |