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Chromosomal Mutagenesis

Overview of attention for book
Cover of 'Chromosomal Mutagenesis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Genome Editing by Targeted Chromosomal Mutagenesis.
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    Chapter 2 piggyBac Transposon-Based Insertional Mutagenesis in Mouse Haploid Embryonic Stem Cells.
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    Chapter 3 Using phage integrases in a site-specific dual integrase cassette exchange strategy.
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    Chapter 4 Therapeutic Genome Mutagenesis Using Synthetic Donor DNA and Triplex-Forming Molecules
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    Chapter 5 Genome Engineering Using Adeno-Associated Virus (AAV).
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    Chapter 6 Engineering of Customized Meganucleases via In Vitro Compartmentalization and In Cellulo Optimization
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    Chapter 7 Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform
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    Chapter 8 Ligation-Independent Cloning (LIC) Assembly of TALEN Genes
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    Chapter 9 Assembly and Characterization of megaTALs for Hyperspecific Genome Engineering Applications
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    Chapter 10 Genome Engineering Using CRISPR-Cas9 System
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    Chapter 11 Donor Plasmid Design for Codon and Single Base Genome Editing Using Zinc Finger Nucleases.
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    Chapter 12 Endogenous Gene Tagging with Fluorescent Proteins.
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    Chapter 13 Silencing Long Noncoding RNAs with Genome-Editing Tools
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    Chapter 14 Gene Editing Using ssODNs with Engineered Endonucleases.
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    Chapter 15 Genome Editing in Human Pluripotent Stem Cells Using Site-Specific Nucleases
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    Chapter 16 Strategies to Increase Genome Editing Frequencies and to Facilitate the Identification of Edited Cells
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    Chapter 17 Using Engineered Endonucleases to Create Knockout and Knockin Zebrafish Models.
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    Chapter 18 Creating Knockout and Knockin Rodents Using Engineered Endonucleases via Direct Embryo Injection.
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    Chapter 19 Simple Sperm Preservation by Freeze-Drying for Conserving Animal Strains
Attention for Chapter 12: Endogenous Gene Tagging with Fluorescent Proteins.
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Chapter title
Endogenous Gene Tagging with Fluorescent Proteins.
Chapter number 12
Book title
Chromosomal Mutagenesis
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-1862-1_12
Pubmed ID
25408409
Book ISBNs
978-1-4939-1861-4, 978-1-4939-1862-1
Authors

John Fetter, Andrey Samsonov, Nathan Zenser, Fan Zhang, Hongyi Zhang, Dmitry Malkov

Abstract

Human genome manipulation has become a powerful tool for understanding the mechanisms of numerous diseases including cancer. Inserting reporter sequences in the desired locations in the genome of a cell can allow monitoring of endogenous activities of disease related genes. Native gene expression and regulation is preserved in these knock-in cells in contrast to cell lines with target overexpression under an exogenous promoter as in the case of transient transfection or stable cell lines with random integration. The fusion proteins created using the modern genome editing tools are expressed at their physiological level and thus are more likely to retain the characteristic expression profile of the endogenous proteins in the cell. Unlike biochemical assays or immunostaining, using a tagged protein under endogenous regulation avoids fixation artifacts and allows detection of the target's activity in live cells. Multiple gene targets could be tagged in a single cell line allowing for the creation of effective cell-based assays for compound screening to discover novel drugs.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 6 21%
Researcher 5 18%
Student > Ph. D. Student 4 14%
Student > Master 3 11%
Student > Doctoral Student 1 4%
Other 3 11%
Unknown 6 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 39%
Agricultural and Biological Sciences 8 29%
Chemistry 2 7%
Neuroscience 1 4%
Unknown 6 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 November 2014.
All research outputs
#18,384,336
of 22,771,140 outputs
Outputs from Methods in molecular biology
#7,867
of 13,090 outputs
Outputs of similar age
#255,662
of 352,903 outputs
Outputs of similar age from Methods in molecular biology
#479
of 996 outputs
Altmetric has tracked 22,771,140 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,903 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.

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