Chapter title |
Proteomic Analysis of Rice Golgi Membranes Isolated by Floating Through Discontinuous Sucrose Density Gradient
|
---|---|
Chapter number | 6 |
Book title |
Plant Membrane Proteomics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7411-5_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7409-2, 978-1-4939-7411-5
|
Authors |
Kazusato Oikawa, Takuya Inomata, Yoshitoshi Hirao, Tadashi Yamamoto, Marouane Baslam, Kentaro Kaneko, Toshiaki Mitsui |
Abstract |
The Golgi apparatus is an endomembrane system organelle and has roles in glycosylation, sorting, and secretion of proteins in the secretory pathway. It has a central function in living organism and is also essential for plant growth. Proteomic approaches to identify the Golgi membrane proteins have been performed in cell suspension cultures and many Golgi membrane-associated proteins were found, whereas it has well established in rice seedling yet. In this chapter, our recent improving published methods for isolated rice Golgi membranes by floating through a discontinuous sucrose density gradient are provided in detail with proteomic analyses. |
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