Chapter title |
Targeted Quantification of Isoforms of a Thylakoid-Bound Protein: MRM Method Development
|
---|---|
Chapter number | 10 |
Book title |
Plant Membrane Proteomics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7411-5_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7409-2, 978-1-4939-7411-5
|
Authors |
Roque Bru-Martínez, Ascensión Martínez-Márquez, Jaime Morante-Carriel, Susana Sellés-Marchart, María José Martínez-Esteso, José Luis Pineda-Lucas, Ignacio Luque, Bru-Martínez, Roque, Martínez-Márquez, Ascensión, Morante-Carriel, Jaime, Sellés-Marchart, Susana, Martínez-Esteso, María José, Pineda-Lucas, José Luis, Luque, Ignacio |
Abstract |
Targeted mass spectrometric methods such as selected/multiple reaction monitoring (SRM/MRM) have found intense application in protein detection and quantification which competes with classical immunoaffinity techniques. It provides a universal procedure to develop a fast, highly specific, sensitive, accurate, and cheap methodology for targeted detection and quantification of proteins based on the direct analysis of their surrogate peptides typically generated by tryptic digestion. This methodology can be advantageously applied in the field of plant proteomics and particularly for non-model species since immunoreagents are scarcely available. Here, we describe the issues to take into consideration in order to develop a MRM method to detect and quantify isoforms of the thylakoid-bound protein polyphenol oxidase from the non-model and database underrepresented species Eriobotrya japonica Lindl. |
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