Chapter title |
Multiparametric Analysis of Apoptosis by Flow Cytometry
|
---|---|
Chapter number | 10 |
Book title |
Flow Cytometry Protocols
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7346-0_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7344-6, 978-1-4939-7346-0
|
Authors |
William G. Telford |
Abstract |
Flow cytometry is the most widely used method for detecting and quantifying apoptosis in mammalian cells. The multiparametric nature of flow cytometry allows several apoptotic characteristics to be combined in a single sample, making it a powerful tool for analyzing the complex progression of apoptotic death. This chapter provides guidelines for combining single-apoptosis assays such as fluorogenic caspase substrates, annexin V binding, DNA dye exclusion, and covalent viability probes into informative multiparametric assays. This multiparametric approach to analyzing apoptosis provides much more information than single-parameter assays that provide only a percentage apoptotic result, given that multiple early, intermediate, and late apoptotic stages can be observed and quantified simultaneously. While much more informative than single-color assays, these multicolor methods can still be analyzed on relatively simple flow cytometers, making them accessible to many laboratories. |
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