| Chapter title |
Multiparameter Conventional Flow Cytometry
|
|---|---|
| Chapter number | 8 |
| Book title |
Flow Cytometry Protocols
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7346-0_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7344-6, 978-1-4939-7346-0
|
| Authors |
Katherine M. McKinnon |
| Abstract |
Multicolor flow cytometry is a useful technique when examining mixed populations of cells, such as blood and tissue cells in human and animal samples. The ability to use multiple fluorescent markers simultaneously allows for the identification of multiple cell types, as well as functional markers that further characterize each sample. The introduction of instruments capable of measuring 12-plus colors and new reagents has made this type of flow cytometry both popular and problematic. Adapting a typical staining panel from 4 to 6 color tubes to more than 12 colors is not simply a matter of "plug and play", but must be approached in a systematic manner to achieve a successful multi-parameter staining panel. This chapter will examine the considerations and methods needed to successfully perform multicolor flow cytometry. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 35 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 17% |
| Researcher | 5 | 14% |
| Student > Ph. D. Student | 5 | 14% |
| Student > Master | 4 | 11% |
| Student > Doctoral Student | 2 | 6% |
| Other | 1 | 3% |
| Unknown | 12 | 34% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 23% |
| Immunology and Microbiology | 5 | 14% |
| Medicine and Dentistry | 3 | 9% |
| Agricultural and Biological Sciences | 3 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Other | 3 | 9% |
| Unknown | 12 | 34% |