Chapter title |
Analysis of Cotranslational Polyamine Sensing During Decoding of ODC Antizyme mRNA
|
---|---|
Chapter number | 26 |
Book title |
Polyamines
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7398-9_26 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7397-2, 978-1-4939-7398-9
|
Authors |
R. Palanimurugan, Daniela Gödderz, Leo Kurian, R. Jürgen Dohmen |
Abstract |
Polyamines are essential poly-cations with vital functions in all cellular systems. Their levels are controlled by intricate regulatory feedback mechanisms. Abnormally high levels of polyamines have been linked to cancer. A rate-limiting enzyme in the biosynthesis of polyamines in fungi and higher eukaryotes is ornithine-decarboxylase (ODC). Its levels are largely controlled posttranslationally via ubiquitin-independent degradation mediated by ODC antizyme (OAZ). The latter is a critical polyamine sensor in a feedback control mechanism that adjusts cellular polyamine levels. Here, we describe an approach employing quantitative western blot analyses that provides in vivo evidence for cotranslational polyamine-sensing by nascent OAZ in yeast. In addition, we describe an in vitro method to detect polyamine binding by antizyme. |
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