Chapter title |
Two Photon Intravital Microscopy of Lyme Borrelia in Mice
|
---|---|
Chapter number | 20 |
Book title |
Borrelia burgdorferi
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7383-5_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7382-8, 978-1-4939-7383-5
|
Authors |
Alexia A. Belperron, Jialing Mao, Linda K. Bockenstedt |
Abstract |
Two-photon intravital microscopy is a powerful tool that allows visualization of cells in intact tissues in a live animal in real time. In recent years, this advanced technology has been applied to understand pathogen-host interactions using fluorescently labeled bacteria. In particular, infectious fluorescent transformants of the Lyme disease spirochete Borrelia burgdorferi, an Ixodes tick-transmitted pathogen, have been imaged by two-photon intravital microscopy to study bacterial motility and interactions of the pathogen with feeding ticks and host tissues. Here, we describe the techniques and equipment used to image mammalian-adapted spirochetes in the skin of living mice in vivo and in joints ex vivo using two-photon intravital microscopy. |
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