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Borrelia burgdorferi

Overview of attention for book
Cover of 'Borrelia burgdorferi'

Table of Contents

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    Book Overview
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    Chapter 1 Genotyping Strains of Lyme Disease Agents Directly From Ticks, Blood, or Tissue
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    Chapter 2 Species Identification and Phylogenetic Analysis of Borrelia burgdorferi Sensu Lato Using Molecular Biological Methods
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    Chapter 3 Generation of Mammalian Host-Adapted Borrelia burgdorferi by Cultivation in Peritoneal Dialysis Membrane Chamber Implantation in Rats
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    Chapter 4 Co-immunoprecipitation for Identifying Protein-Protein Interactions in Borrelia burgdorferi
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    Chapter 5 Characterization of Borrelia burgdorferi Binding to Mammalian Cells and Extracellular Matrix
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    Chapter 6 Analysis of Lipids and Lipid Rafts in Borrelia
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    Chapter 7 Trace Element Analysis of Borrelia burgdorferi by Inductively Coupled Plasma-Sector Field Mass Spectrometry
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    Chapter 8 Identification and Characterization of Borrelia burgdorferi Complement-Binding Proteins
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    Chapter 9 Artificial Infection of Ticks with Borrelia burgdorferi Using a Microinjection Method and Their Detection In Vivo Using Quantitative PCR Targeting flaB RNA
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    Chapter 10 Borrelia burgdorferi Microarray Analysis
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    Chapter 11 Borrelia burgdorferi Transcriptome Analysis by RNA-Sequencing
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    Chapter 12 Selection of Borrelia burgdorferi Promoter Sequences Active During Mammalian Infection Using In Vivo Expression Technology
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    Chapter 13 Analysis of DNA and RNA Binding Properties of Borrelia burgdorferi Regulatory Proteins
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    Chapter 14 Identification of Acetylated Proteins in Borrelia burgdorferi
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    Chapter 15 Genetic Transformation and Complementation
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    Chapter 16 Genome-Wide Mutagenesis in Borrelia burgdorferi
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    Chapter 17 Generation of Conditional Mutants in Borrelia burgdorferi
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    Chapter 18 Detection of Bioluminescent Borrelia burgdorferi from In Vitro Cultivation and During Murine Infection
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    Chapter 19 Analysis of Borrelia burgdorferi Proteome and Protein–Protein Interactions
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    Chapter 20 Two Photon Intravital Microscopy of Lyme Borrelia in Mice
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    Chapter 21 Investigating Human Dendritic Cell Immune Responses to Borrelia burgdorferi
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    Chapter 22 Phagocytosis Assays for Borrelia burgdorferi
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    Chapter 23 Measuring Borrelia burgdorferi Motility and Chemotaxis
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    Chapter 24 In Vitro Models of Cutaneous Inflammation
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    Chapter 25 Magnetic Isolation of Phagosomes Containing Toll-Like Receptor Ligands
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    Chapter 26 Xenodiagnosis Using Ixodes scapularis Larval Ticks in Humans
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    Chapter 27 Recombinant E. coli Dualistic Role as an Antigen-adjuvant Delivery Vehicle for Oral Immunization
Attention for Chapter 12: Selection of Borrelia burgdorferi Promoter Sequences Active During Mammalian Infection Using In Vivo Expression Technology
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Chapter title
Selection of Borrelia burgdorferi Promoter Sequences Active During Mammalian Infection Using In Vivo Expression Technology
Chapter number 12
Book title
Borrelia burgdorferi
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7383-5_12
Pubmed ID
Book ISBNs
978-1-4939-7382-8, 978-1-4939-7383-5
Authors

Philip P. Adams, Mollie W. Jewett

Abstract

In vivo expression technology (IVET) has been applied to a variety of organisms to identify active promoters in specific environments or growth conditions of interest. Here, we describe modifications to employ this genome-wide screening method for Borrelia burgdorferi, the Lyme disease spirochete, during an active murine infection. Utilization of this technique provides valuable insights into the B. burgdorferi transcriptome during infection, despite the low bacterial numbers in the mammalian host environment.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 40%
Student > Master 2 20%
Researcher 2 20%
Professor 1 10%
Unknown 1 10%
Readers by discipline Count As %
Immunology and Microbiology 5 50%
Agricultural and Biological Sciences 2 20%
Veterinary Science and Veterinary Medicine 1 10%
Medicine and Dentistry 1 10%
Unknown 1 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 December 2017.
All research outputs
#14,366,228
of 23,006,268 outputs
Outputs from Methods in molecular biology
#4,222
of 13,160 outputs
Outputs of similar age
#240,399
of 442,254 outputs
Outputs of similar age from Methods in molecular biology
#432
of 1,498 outputs
Altmetric has tracked 23,006,268 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,160 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,254 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,498 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.