Chapter title |
Trace Element Analysis of Borrelia burgdorferi by Inductively Coupled Plasma-Sector Field Mass Spectrometry
|
---|---|
Chapter number | 7 |
Book title |
Borrelia burgdorferi
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7383-5_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7382-8, 978-1-4939-7383-5
|
Authors |
Xin Li, Peng Wang, Anthony Lutton, John Olesik |
Abstract |
Transition metal ions play important structural, regulatory, and catalytic roles in all biological systems by serving as cofactors for proteins. Due to their relatively low levels in the cell compared to abundant metal ions such as potassium and magnesium, transition metals are often considered micronutrients and referred to as trace elements. Manganese (Mn), iron (Fe), copper (Cu), and zinc (Zn) are the most prevalent transition metals in the Lyme disease spirochete Borrelia burgdorferi. Here, we describe a method for the accurate measurement of these trace elements in B. burgdorferi utilizing inductively coupled plasma-sector field mass spectrometry (ICP-SFMS). |
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