Chapter title |
Exporters for Production of Amino Acids and Other Small Molecules
|
---|---|
Chapter number | 32 |
Book title |
Amino Acid Fermentation
|
Published in |
Advances in biochemical engineering biotechnology, January 2016
|
DOI | 10.1007/10_2016_32 |
Pubmed ID | |
Book ISBNs |
978-4-43-156518-5, 978-4-43-156520-8
|
Authors |
Lothar Eggeling, Eggeling, Lothar |
Abstract |
Microbes are talented catalysts to synthesize valuable small molecules in their cytosol. However, to make full use of their skills - and that of metabolic engineers - the export of intracellularly synthesized molecules to the culture medium has to be considered. This step is as essential as is each step for the synthesis of the favorite molecule of the metabolic engineer, but is frequently not taken into account. To export small molecules via the microbial cell envelope, a range of different types of carrier proteins is recognized to be involved, which are primary active carriers, secondary active carriers, or proteins increasing diffusion. Relevant export may require just one carrier as is the case with L-lysine export by Corynebacterium glutamicum or involve up to four carriers as known for L-cysteine excretion by Escherichia coli. Meanwhile carriers for a number of small molecules of biotechnological interest are recognized, like for production of peptides, nucleosides, diamines, organic acids, or biofuels. In addition to carriers involved in amino acid excretion, such carriers and their impact on product formation are described, as well as the relatedness of export carriers which may serve as a hint to identify further carriers required to improve product formation by engineering export. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 4% |
Germany | 1 | 4% |
Unknown | 24 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 19% |
Other | 3 | 12% |
Researcher | 3 | 12% |
Student > Master | 3 | 12% |
Professor | 2 | 8% |
Other | 1 | 4% |
Unknown | 9 | 35% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 9 | 35% |
Immunology and Microbiology | 3 | 12% |
Biochemistry, Genetics and Molecular Biology | 2 | 8% |
Engineering | 1 | 4% |
Unknown | 11 | 42% |