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Chromatin Immunoprecipitation

Overview of attention for book
Cover of 'Chromatin Immunoprecipitation'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 ChIP and ChIP-Related Techniques: Expanding the Fields of Application and Improving ChIP Performance
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    Chapter 2 Considerations on Experimental Design and Data Analysis of Chromatin Immunoprecipitation Experiments
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    Chapter 3 How to Combine ChIP with qPCR
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    Chapter 4 Analysis of Protein–DNA Interaction by Chromatin Immunoprecipitation and DNA Tiling Microarray (ChIP-on-chip)
  6. Altmetric Badge
    Chapter 5 Chromatin Immunoprecipitation from Mouse Embryonic Tissue or Adherent Cells in Culture, Followed by Next-Generation Sequencing
  7. Altmetric Badge
    Chapter 6 Chromatin RNA Immunoprecipitation (ChRIP)
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    Chapter 7 DNA Accessibility by MNase Digestions
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    Chapter 8 Characterization of the Nucleosome Landscape by Micrococcal Nuclease-Sequencing (MNase-seq)
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    Chapter 9 ChIP-re-ChIP: Co-occupancy Analysis by Sequential Chromatin Immunoprecipitation
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    Chapter 10 Sm-ChIPi: Single-Molecule Chromatin Immunoprecipitation Imaging
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    Chapter 11 Chromatin Immunoprecipitation of Skeletal Muscle Tissue
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    Chapter 12 Chromatin Immunoprecipitation Assay in the Hyperthermoacidophilic Crenarchaeon, Sulfolobus acidocaldarius
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    Chapter 13 Using Intra-ChIP to Measure Protein–DNA Interactions in Intracellular Pathogens
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    Chapter 14 Native Chromatin Immunoprecipitation-Sequencing (ChIP-Seq) from Low Cell Numbers
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    Chapter 15 MOBE-ChIP: Probing Cell Type-Specific Binding Through Large-Scale Chromatin Immunoprecipitation
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    Chapter 16 Multiplexed ChIP-Seq Using Direct Nucleosome Barcoding: A Tool for High-Throughput Chromatin Analysis
  18. Altmetric Badge
    Chapter 17 Analysis of ChIP-seq Data in R/Bioconductor
  19. Altmetric Badge
    Chapter 18 Spike-In Normalization of ChIP Data Using DNA–DIG–Antibody Complex
Attention for Chapter 7: DNA Accessibility by MNase Digestions
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Chapter title
DNA Accessibility by MNase Digestions
Chapter number 7
Book title
Chromatin Immunoprecipitation
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7380-4_7
Pubmed ID
Book ISBNs
978-1-4939-7379-8, 978-1-4939-7380-4
Authors

Ann-Kristin Östlund Farrants

Abstract

Micrococcal nuclease (MNase) digestion of chromatin cuts linker DNA between neighboring nucleosomes and in this way generates mononucleosomes. The protected fragments can then be analyzed by genome-wide sequencing techniques or by quantitative PCR to obtain information about the positions of nucleosomes in the chromatin. Nucleosomes are differentially sensitive to MNase digestion, which means that titrations of MNase should be performed to obtain a comprehensive map of the nucleosome positions of a chromatin region or genome.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 5 45%
Student > Master 2 18%
Researcher 2 18%
Student > Ph. D. Student 1 9%
Unknown 1 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 45%
Agricultural and Biological Sciences 4 36%
Unknown 2 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 October 2017.
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#20,449,496
of 23,005,189 outputs
Outputs from Methods in molecular biology
#9,941
of 13,160 outputs
Outputs of similar age
#378,088
of 442,254 outputs
Outputs of similar age from Methods in molecular biology
#1,193
of 1,498 outputs
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