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Advancements of Mass Spectrometry in Biomedical Research

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Cover of 'Advancements of Mass Spectrometry in Biomedical Research'

Table of Contents

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    Book Overview
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    Chapter 1 Mass spectrometry for proteomics-based investigation.
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    Chapter 2 MALDI Profiling and Applications in Medicine
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    Chapter 3 Simplifying the proteome: analytical strategies for improving peak capacity.
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    Chapter 4 Quantitative shotgun proteomics with data-independent acquisition and traveling wave ion mobility spectrometry: a versatile tool in the life sciences.
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    Chapter 5 Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Quantitative Proteomics.
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    Chapter 6 Utility of computational structural biology in mass spectrometry.
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    Chapter 7 Affinity-Mass Spectrometry Approaches for Elucidating Structures and Interactions of Protein–Ligand Complexes
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    Chapter 8 Neurological Analyses: Focus on Gangliosides and Mass Spectrometry
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    Chapter 9 Mass Spectrometric Analysis of Post-translational Modifications (PTMs) and Protein-Protein Interactions (PPIs).
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    Chapter 10 Applications for Mass Spectrometry in the Study of Ion Channel Structure and Function
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    Chapter 11 A Mass Spectrometry View of Stable and Transient Protein Interactions
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    Chapter 12 Mass Spectrometry-Based Tissue Imaging of Small Molecules
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    Chapter 13 Redox proteomics: from bench to bedside.
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    Chapter 14 Analysis of Fluorinated Proteins by Mass Spectrometry
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    Chapter 15 Mass spectrometry for proteomics-based investigation using the zebrafish vertebrate model system.
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    Chapter 16 Mass Spectrometry-Based Biomarkers in Drug Development
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    Chapter 17 Detection of Biomedically Relevant Stilbenes from Wines by Mass Spectrometry
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    Chapter 18 Mass Spectrometric DNA Adduct Quantification by Multiple Reaction Monitoring and Its Future Use for the Molecular Epidemiology of Cancer
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    Chapter 19 Using Breast Milk to Assess Breast Cancer Risk: The Role of Mass Spectrometry-Based Proteomics
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    Chapter 20 Cancer secretomes and their place in supplementing other hallmarks of cancer.
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    Chapter 21 Thiostrepton, a natural compound that triggers heat shock response and apoptosis in human cancer cells: a proteomics investigation.
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    Chapter 22 Using Proteomics to Unravel the Mysterious Steps of the HBV-Life-Cycle.
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    Chapter 23 Oxidative Stress and Antibiotic Resistance in Bacterial Pathogens: State of the Art, Methodologies, and Future Trends
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    Chapter 24 Proteomic approaches to dissect neuronal signaling pathways.
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    Chapter 25 Investigating a Novel Protein Using Mass Spectrometry: The Example of Tumor Differentiation Factor (TDF).
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    Chapter 26 Mass spectrometry for the study of autism and neurodevelopmental disorders.
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    Chapter 27 Biomarkers in major depressive disorder: the role of mass spectrometry.
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    Chapter 28 Application of Mass Spectrometry to Characterize Localization and Efficacy of Nanoceria In Vivo
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    Chapter 29 Bottlenecks in proteomics.
Attention for Chapter 5: Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Quantitative Proteomics.
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Chapter title
Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Quantitative Proteomics.
Chapter number 5
Book title
Advancements of Mass Spectrometry in Biomedical Research
Published in
Advances in experimental medicine and biology, June 2014
DOI 10.1007/978-3-319-06068-2_5
Pubmed ID
Book ISBNs
978-3-31-906067-5, 978-3-31-906068-2
Authors

Hoedt E, Zhang G, Neubert TA, Esthelle Hoedt, Guoan Zhang, Thomas A. Neubert

Abstract

Stable isotope labeling by amino acids in cell culture (SILAC) is a powerful approach for high-throughput quantitative proteomics. SILAC allows highly accurate protein quantitation through metabolic encoding of whole cell proteomes using stable isotope labeled amino acids. Since its introduction in 2002, SILAC has become increasingly popular. In this chapter we review the methodology and application of SILAC, with an emphasis on three research areas: dynamics of posttranslational modifications, protein-protein interactions, and protein turnover.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 2 4%
Canada 1 2%
Unknown 51 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 19%
Researcher 10 19%
Student > Master 9 17%
Student > Postgraduate 4 7%
Professor > Associate Professor 4 7%
Other 8 15%
Unknown 9 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 16 30%
Agricultural and Biological Sciences 10 19%
Medicine and Dentistry 5 9%
Chemistry 4 7%
Pharmacology, Toxicology and Pharmaceutical Science 2 4%
Other 6 11%
Unknown 11 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 October 2014.
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#18,379,655
of 22,765,347 outputs
Outputs from Advances in experimental medicine and biology
#3,304
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Outputs of similar age
#163,992
of 228,095 outputs
Outputs of similar age from Advances in experimental medicine and biology
#29
of 42 outputs
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