Chapter title |
Mass spectrometry for proteomics-based investigation using the zebrafish vertebrate model system.
|
---|---|
Chapter number | 15 |
Book title |
Advancements of Mass Spectrometry in Biomedical Research
|
Published in |
Advances in experimental medicine and biology, June 2014
|
DOI | 10.1007/978-3-319-06068-2_15 |
Pubmed ID | |
Book ISBNs |
978-3-31-906067-5, 978-3-31-906068-2
|
Authors |
Baral R, Wetie AG, Darie CC, Wallace KN, Reshica Baral, Armand G. Ngounou Wetie, Costel C. Darie, Kenneth N. Wallace |
Abstract |
The zebrafish (Danio rerio) is frequently being used to investigate the genetics of human diseases as well as resulting pathologies. Ease of both forward and reverse genetic manipulation along with conservation of vertebrate organ systems and disease causing genes has made this system a popular model. Many techniques have been developed to manipulate the genome of zebrafish producing mutants in a vast array of genes. While genetic manipulation of zebrafish has progressed, proteomics have been under-utilized. This review highlights studies that have already been performed using proteomic techniques and as well as our initial proteomic work comparing changes to the proteome between the ascl1a-/- and WT intestine. |
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