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Molecular Methods for Evolutionary Genetics

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Cover of 'Molecular Methods for Evolutionary Genetics'

Table of Contents

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    Book Overview
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    Chapter 1 Genome Size Determination Using Flow Cytometry of Propidium Iodide-Stained Nuclei
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    Chapter 2 Chromosome Analysis in Invertebrates and Vertebrates
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    Chapter 3 Genomic libraries: I. Construction and screening of fosmid genomic libraries.
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    Chapter 4 Genomic Libraries: II. Subcloning, Sequencing, and Assembling Large-Insert Genomic DNA Clones.
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    Chapter 5 Reduced Representation Methods for Subgenomic Enrichment and Next-Generation Sequencing
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    Chapter 6 Accessing the Transcriptome: How to Normalize mRNA Pools
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    Chapter 7 Transcriptome Sequencing Goals, Assembly, and Assessment
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    Chapter 8 Rapid retrieval of DNA target sequences by primer extension capture.
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    Chapter 9 SNP Discovery and Genotyping for Evolutionary Genetics Using RAD Sequencing.
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    Chapter 10 DNA Microarray-Based Mutation Discovery and Genotyping
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    Chapter 11 Genotyping with Sequenom
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    Chapter 12 Isolating Microsatellite Loci: Looking Back, Looking Ahead
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    Chapter 13 Design of Custom Oligonucleotide Microarrays for Single Species or Interspecies Hybrids Using Array Oligo Selector
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    Chapter 14 Identification of Homologous Gene Sequences by PCR with Degenerate Primers
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    Chapter 15 Characterizing cDNA Ends by Circular RACE
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    Chapter 16 Identification of DNA Sequences that Flank a Known Region by Inverse PCR
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    Chapter 17 Quantification of Transcript Levels with Quantitative RT-PCR
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    Chapter 18 Using Pyrosequencing to Measure Allele-Specific mRNA Abundance and Infer the Effects of Cis- and Trans-regulatory Differences
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    Chapter 19 Whole-Mount In Situ Hybridization of Sectioned Tissues of Species Hybrids to Detect Cis -regulatory Changes in Gene Expression Pattern
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    Chapter 20 Identifying Fluorescently Labeled Single Molecules in Image Stacks Using Machine Learning
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    Chapter 21 Experimental Approaches to Evaluate the Contributions of Candidate Cis- regulatory Mutations to Phenotypic Evolution
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    Chapter 22 Experimental Approaches to Evaluate the Contributions of Candidate Protein-Coding Mutations to Phenotypic Evolution
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    Chapter 23 Making Reporter Gene Constructs to Analyze Cis -regulatory Elements
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    Chapter 24 PCR-Directed In Vivo Plasmid Construction Using Homologous Recombination in Baker’s Yeast
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    Chapter 25 Production of Fosmid Genomic Libraries Optimized for Liquid Culture Recombineering and Cross-Species Transgenesis
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    Chapter 26 Recombination-Mediated Genetic Engineering of Large Genomic DNA Transgenes
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    Chapter 27 Overlap Extension PCR: An Efficient Method for Transgene Construction
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    Chapter 28 Gene Knockdown Analysis by Double-Stranded RNA Injection
Attention for Chapter 9: SNP Discovery and Genotyping for Evolutionary Genetics Using RAD Sequencing.
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Chapter title
SNP Discovery and Genotyping for Evolutionary Genetics Using RAD Sequencing.
Chapter number 9
Book title
Molecular Methods for Evolutionary Genetics
Published in
Methods in molecular biology, January 2012
DOI 10.1007/978-1-61779-228-1_9
Pubmed ID
Book ISBNs
978-1-61779-227-4, 978-1-61779-228-1
Authors

Etter PD, Bassham S, Hohenlohe PA, Johnson EA, Cresko WA, Paul D. Etter, Susan Bassham, Paul A. Hohenlohe, Eric A. Johnson, William A. Cresko, Etter, Paul D., Bassham, Susan, Hohenlohe, Paul A., Johnson, Eric A., Cresko, William A.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 474 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 10 2%
United Kingdom 4 <1%
Australia 2 <1%
Italy 2 <1%
Spain 2 <1%
Netherlands 1 <1%
Norway 1 <1%
France 1 <1%
Uganda 1 <1%
Other 8 2%
Unknown 442 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 113 24%
Researcher 103 22%
Student > Master 78 16%
Student > Bachelor 39 8%
Student > Doctoral Student 23 5%
Other 59 12%
Unknown 59 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 280 59%
Biochemistry, Genetics and Molecular Biology 64 14%
Environmental Science 28 6%
Immunology and Microbiology 4 <1%
Psychology 4 <1%
Other 20 4%
Unknown 74 16%