Chapter title |
Genomic libraries: I. Construction and screening of fosmid genomic libraries.
|
---|---|
Chapter number | 3 |
Book title |
Molecular Methods for Evolutionary Genetics
|
Published in |
Methods in molecular biology, July 2011
|
DOI | 10.1007/978-1-61779-228-1_3 |
Pubmed ID | |
Book ISBNs |
978-1-61779-227-4, 978-1-61779-228-1
|
Authors |
Quail MA, Matthews L, Sims S, Lloyd C, Beasley H, Baxter SW, Quail, Mike A., Matthews, Lucy, Sims, Sarah, Lloyd, Christine, Beasley, Helen, Baxter, Simon W., Mike A. Quail, Lucy Matthews, Sarah Sims, Christine Lloyd, Helen Beasley, Simon W. Baxter |
Abstract |
Large insert genome libraries have been a core resource required to sequence genomes, analyze haplotypes, and aid gene discovery. While next generation sequencing technologies are revolutionizing the field of genomics, traditional genome libraries will still be required for accurate genome assembly. Their utility is also being extended to functional studies for understanding DNA regulatory elements. Here, we present a detailed method for constructing genomic fosmid libraries, testing for common contaminants, gridding the library to nylon membranes, then hybridizing the library membranes with a radiolabeled probe to identify corresponding genomic clones. While this chapter focuses on fosmid libraries, many of these steps can also be applied to bacterial artificial chromosome libraries. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 22% |
Researcher | 3 | 17% |
Student > Bachelor | 3 | 17% |
Student > Master | 2 | 11% |
Student > Doctoral Student | 1 | 6% |
Other | 1 | 6% |
Unknown | 4 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 22% |
Biochemistry, Genetics and Molecular Biology | 3 | 17% |
Chemical Engineering | 1 | 6% |
Business, Management and Accounting | 1 | 6% |
Computer Science | 1 | 6% |
Other | 2 | 11% |
Unknown | 6 | 33% |