Chapter title |
Proteomic Analysis of Secreted Proteins from Cell Microenvironment
|
---|---|
Chapter number | 4 |
Book title |
Plant Protein Secretion
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7262-3_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7261-6, 978-1-4939-7262-3
|
Authors |
Subash Adhikari, Lan Chen, Peiwu Huang, Ruijun Tian |
Abstract |
Cell microenvironment consists of various types of cells which communicate with each other by vast number of secreted proteins. An unbiased profiling of these secreted proteins on a global scale is often critical for understanding the intercellular signaling in an autocrine or paracrine manner. Mass spectrometry-based proteomics has become one of the most popular technology for characterization of the secreted proteins. In this chapter, we discuss the standard workflow for secreted proteins characterization, including harvesting secreted proteins from conditioned media, digesting the obtained proteins, liquid chromatography-mass spectrometry analysis, and downstream data analysis. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 36% |
Unspecified | 1 | 9% |
Student > Ph. D. Student | 1 | 9% |
Professor | 1 | 9% |
Student > Master | 1 | 9% |
Other | 1 | 9% |
Unknown | 2 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 45% |
Unspecified | 1 | 9% |
Computer Science | 1 | 9% |
Agricultural and Biological Sciences | 1 | 9% |
Unknown | 3 | 27% |