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Chapter title |
The Organelle pH Estimate and Measurement in Plant Secretory Pathway
|
---|---|
Chapter number | 20 |
Book title |
Plant Protein Secretion
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-7262-3_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7261-6, 978-1-4939-7262-3
|
Authors |
Jinbo Shen |
Abstract |
The maintenance of pH in the intracellular compartments is essential for the viability of cells. Several genetically encoded fluorescent pH sensors have been developed for noninvasive in vivo analysis. Here, we describe the methods using PEpHluorin (plant-solubility-modified ecliptic pHluorin) and PRpHluorin (plant-solubility-modified ratiometric pHluorin) as pH sensors for in vivo visualization and quantification organelle pH of in plant secretory pathway. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 2 | 40% |
Researcher | 2 | 40% |
Student > Ph. D. Student | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 80% |
Agricultural and Biological Sciences | 1 | 20% |