Chapter title |
Isolation of the Plant Exocyst Complex
|
---|---|
Chapter number | 22 |
Book title |
Plant Protein Secretion
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7262-3_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7261-6, 978-1-4939-7262-3
|
Authors |
King Pong Leung, Wilson Chun Yu Lau |
Abstract |
The exocyst, conserved from yeast to plants to mammals, is a hetero-octameric complex that mediates tethering of secretory vesicles to designated sites on the plasma membrane during polarized exocytosis. Because structural studies of the intact exocyst complex have been greatly limited by the low yields of purified proteins, many aspects of the exocyst functions remain poorly understood. Here, we present the protocols for the isolation and purification of the recombinant and the native plant exocyst complex. Given the known diversification of the exocyst subunits in plants, our protocols will likely open the possibility of unraveling the functional significance of these subunits in the context of the fully assembled exocyst complex. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 1 | 33% |
Unknown | 2 | 67% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Unknown | 2 | 67% |