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Plant Protein Secretion

Overview of attention for book
Cover of 'Plant Protein Secretion'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 An Overview of Protein Secretion in Yeast and Animal Cells
  3. Altmetric Badge
    Chapter 2 An Overview of Protein Secretion in Plant Cells
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    Chapter 3 Bioinformatics Analysis of Protein Secretion in Plants
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    Chapter 4 Proteomic Analysis of Secreted Proteins from Cell Microenvironment
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    Chapter 5 Using Homology Modeling to Understand the Structural Basis of Specific Interaction of a Plant-Specific AtSar1a–AtSec23a Pair Involved in Protein ER Export
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    Chapter 6 Analysis of Golgi-Mediated Protein Traffic in Plant Cells
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    Chapter 7 Analysis of Membrane Protein Topology in the Plant Secretory Pathway
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    Chapter 8 Semiautomatic Segmentation of Plant Golgi Stacks in Electron Tomograms Using 3dmod
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    Chapter 9 3D Printing of Plant Golgi Stacks from Their Electron Tomographic Models
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    Chapter 10 Transient Expression of Chimeric Fluorescent Reporter Proteins in Pollen Tubes to Study Protein Polar Secretion and Dynamics
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    Chapter 11 Analysis of Actin-Based Intracellular Trafficking in Pollen Tubes
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    Chapter 12 Analysis of Phragmoplast Kinetics During Plant Cytokinesis
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    Chapter 13 Immunofluorescence Analysis of Membrane-Associated Proteins for Clathrin-Mediated Endocytosis in Plant Root Cells
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    Chapter 14 In Vivo Interaction Studies by Measuring Förster Resonance Energy Transfer Through Fluorescence Lifetime Imaging Microscopy (FRET/FLIM)
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    Chapter 15 Analysis of Nanobody–Epitope Interactions in Living Cells via Quantitative Protein Transport Assays
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    Chapter 16 A Secretion System for Cargo Protein Identification of Vacuolar Sorting Receptors
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    Chapter 17 Identifying Novel Regulators of Vacuolar Trafficking by Combining Fluorescence Imaging-Based Forward Genetic Screening and In Vitro Pollen Germination
  19. Altmetric Badge
    Chapter 18 Measuring Plant Protein Secretion
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    Chapter 19 Transient Secretory Enzyme Expression in Leaf Protoplasts to Characterize SNARE Functional Classes in Conventional and Unconventional Secretion
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    Chapter 20 The Organelle pH Estimate and Measurement in Plant Secretory Pathway
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    Chapter 21 Analysis of Exocyst-Positive Organelle (EXPO)-Mediated Unconventional Protein Secretion (UPS) in Plant Cells
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    Chapter 22 Isolation of the Plant Exocyst Complex
  24. Altmetric Badge
    Chapter 23 Using Microscopy Tools to Visualize Autophagosomal Structures in Plant Cells
  25. Altmetric Badge
    Chapter 24 Analysis of Plant Autophagy
Attention for Chapter 22: Isolation of the Plant Exocyst Complex
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Chapter title
Isolation of the Plant Exocyst Complex
Chapter number 22
Book title
Plant Protein Secretion
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7262-3_22
Pubmed ID
Book ISBNs
978-1-4939-7261-6, 978-1-4939-7262-3
Authors

King Pong Leung, Wilson Chun Yu Lau

Abstract

The exocyst, conserved from yeast to plants to mammals, is a hetero-octameric complex that mediates tethering of secretory vesicles to designated sites on the plasma membrane during polarized exocytosis. Because structural studies of the intact exocyst complex have been greatly limited by the low yields of purified proteins, many aspects of the exocyst functions remain poorly understood. Here, we present the protocols for the isolation and purification of the recombinant and the native plant exocyst complex. Given the known diversification of the exocyst subunits in plants, our protocols will likely open the possibility of unraveling the functional significance of these subunits in the context of the fully assembled exocyst complex.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 1 33%
Unknown 2 67%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Unknown 2 67%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 September 2017.
All research outputs
#20,446,373
of 23,001,641 outputs
Outputs from Methods in molecular biology
#9,934
of 13,154 outputs
Outputs of similar age
#356,140
of 421,214 outputs
Outputs of similar age from Methods in molecular biology
#842
of 1,074 outputs
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