| Chapter title |
In Vitro Culture and Phytochemical Analysis of Passiflora tenuifila Killip and Passiflora setacea DC (Passifloraceae)
|
|---|---|
| Chapter number | 2 |
| Book title |
Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3332-7_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3330-3, 978-1-4939-3332-7
|
| Authors |
Jenny Sumara Sozo, Daniel Cuzziol Cruz, Ana Flavia Pavei, Isadora Medeiros da Costa Pereira, Marcia Wolfart, Fernanda Ramlov, Daiane Fiuza Montagner, Marcelo Maraschin, Ana Maria Viana, Sozo, Jenny Sumara, Cruz, Daniel Cuzziol, Pavei, Ana Flavia, Pereira, Isadora Medeiros da Costa, Wolfart, Marcia, Ramlov, Fernanda, Montagner, Daiane Fiuza, Maraschin, Marcelo, Viana, Ana Maria |
| Abstract |
We have developed reproducible micropropagation, callus culture, phytochemical, and antioxidant analysis protocols for the wild passion fruit species P. tenuifila, and P. setacea, native to the Brazilian endangered biomes Atlantic Forest, Cerrado, and Caatinga, by using seeds and explants from seedlings and adult plants. Genotype and explant origin-linked differences are visible amongst the Passiflora species concerning callus production, total phenolics, and antioxidant activity. The protocols developed for screening phytochemicals and antioxidants in P. tenuifila and P. setacea callus extracts have shown their potential for phenolic production and antioxidant activity. The high level of phenolic compounds seems to account for the antioxidant activity of methanolic extracts of P. tenuifila derived from 45-day-old immature seed callus. The methanolic extracts of callus derived from P. setacea seedling leaf node and cotyledonary node explants have shown the highest antioxidant activity despite their lower content of phenolics, as compared to cotyledon callus extracts. The optimized micropropagation and callus culture protocols have great potential to use cell culture techniques for further vegetative propagation, in vitro germplasm conservation, and secondary metabolite production using biotic and abiotic elicitors. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 19% |
| Student > Doctoral Student | 2 | 10% |
| Student > Master | 2 | 10% |
| Unspecified | 1 | 5% |
| Student > Ph. D. Student | 1 | 5% |
| Other | 3 | 14% |
| Unknown | 8 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 33% |
| Biochemistry, Genetics and Molecular Biology | 2 | 10% |
| Environmental Science | 1 | 5% |
| Nursing and Health Professions | 1 | 5% |
| Unspecified | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 9 | 43% |