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Chapter title |
Preparation of Disease-Related Protein Assemblies for Single Particle Electron Microscopy
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Chapter number | 12 |
Book title |
Proteomics for Drug Discovery
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-7201-2_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7200-5, 978-1-4939-7201-2
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Authors |
A. Cameron Varano, Naoe Harafuji, William Dearnaley, Lisa Guay-Woodford, Deborah F. Kelly |
Abstract |
Electron microscopy (EM) is a rapidly growing area of structural biology that permits us to decode biological assemblies at the nanoscale. To examine biological materials for single particle EM analysis, purified assemblies must be obtained using biochemical separation techniques. Here, we describe effective methodologies for isolating histidine (his)-tagged protein assemblies from the nucleus of disease-relevant cell lines. We further demonstrate how isolated assemblies are visualized using single particle EM techniques and provide representative results for each step in the process. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 2 | 67% |
Student > Ph. D. Student | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Agricultural and Biological Sciences | 1 | 33% |
Medicine and Dentistry | 1 | 33% |