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Mendeley readers
| Chapter title |
Identification of Lipid Binding Modulators Using the Protein-Lipid Overlay Assay
|
|---|---|
| Chapter number | 13 |
| Book title |
Proteomics for Drug Discovery
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7201-2_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7200-5, 978-1-4939-7201-2
|
| Authors |
Tuo-Xian Tang, Wen Xiong, Carla V. Finkielstein, Daniel G. S. Capelluto |
| Abstract |
The protein-lipid overlay assay is an inexpensive, easy-to-implement, and high-throughput methodology that employs nitrocellulose membranes to immobilize lipids in order to rapid screen and identify protein-lipid interactions. In this chapter, we show how this methodology can identify potential modulators of protein-lipid interactions by screening water-soluble lipid competitors or even the introduction of pH changes during the binding assay to identify pH-dependent lipid binding events. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 23% |
| Professor > Associate Professor | 2 | 15% |
| Student > Master | 2 | 15% |
| Student > Ph. D. Student | 2 | 15% |
| Other | 1 | 8% |
| Other | 2 | 15% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 54% |
| Chemistry | 2 | 15% |
| Agricultural and Biological Sciences | 1 | 8% |
| Medicine and Dentistry | 1 | 8% |
| Unknown | 2 | 15% |