Chapter title |
Methods to Visualize MAVS Subcellular Localization
|
---|---|
Chapter number | 7 |
Book title |
Innate Antiviral Immunity
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7237-1_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7236-4, 978-1-4939-7237-1
|
Authors |
Christine Vazquez, Dia C. Beachboard, Stacy M. Horner, Vazquez, Christine, Beachboard, Dia C., Horner, Stacy M. |
Abstract |
The mitochondrial antiviral signaling (MAVS) protein is a central adaptor protein required for antiviral innate immune signaling. To facilitate its roles in innate immunity, MAVS localizes to multiple intracellular membranous compartments, including the mitochondria, the mitochondrial-associated ER membrane (MAM), and peroxisomes. Studies of MAVS function therefore often require an analysis of MAVS localization. To detect MAVS protein on intracellular membranes, biochemical fractionation to isolate MAMs, mitochondria, or peroxisomes can be used. Further, immunofluorescence with antibodies against specific membrane markers can be used to visualize MAVS distribution throughout the cell. Here, we describe the biochemical fractionation and immunofluorescence protocols used to detect MAVS subcellular localization. |
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