Chapter title |
The chemosensitive response of neurons from the locus coeruleus (LC) to hypercapnic acidosis with clamped intracellular pH.
|
---|---|
Chapter number | 58 |
Book title |
Integration in Respiratory Control
|
Published in |
Advances in experimental medicine and biology, January 2008
|
DOI | 10.1007/978-0-387-73693-8_58 |
Pubmed ID | |
Book ISBNs |
978-0-387-73692-1, 978-0-387-73693-8
|
Authors |
Lynn K Hartzler, Jay B Dean, Robert W Putnam, Lynn K. Hartzler, Jay B. Dean, Robert W. Putnam, Hartzler, Lynn K., Dean, Jay B., Putnam, Robert W. |
Abstract |
Currently, a change in pH(i) is believed to be the major signal in the chemosensitive (CS) response of brainstem neurons to hypercapnia; however, multiple factors (e.g., Ca2+, CO2, pH(i), and pHo) have been suggested to contribute to this increase in firing rate. While there is evidence for a significant role of pH(i) in the CS response, we hypothesize that hypercapnic acidosis (HA) can increase firing rate even with no change in pH(i). We tested several methods to clamp pH(i), including high intracellular buffer and the use of rapid diffusion of weak bases or weak acids through the cell membrane. We were able to clamp pH(i) during hypercapnic exposure using weak acids. We observed a CS response to HA, with pH(i) clamped, indicating that intracellular acidification, while sufficient to increase firing rate, is not required for the response of CS neurons. The CS response to HA without a change in pH(i) is most likely due to extracellular acidification and/or increased CO2 and strongly supports the multiple factors model of chemosensitive signaling. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 5% |
Unknown | 18 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 26% |
Student > Bachelor | 2 | 11% |
Professor > Associate Professor | 2 | 11% |
Librarian | 1 | 5% |
Professor | 1 | 5% |
Other | 3 | 16% |
Unknown | 5 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 3 | 16% |
Agricultural and Biological Sciences | 3 | 16% |
Medicine and Dentistry | 2 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Veterinary Science and Veterinary Medicine | 1 | 5% |
Other | 2 | 11% |
Unknown | 7 | 37% |