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Mendeley readers
Chapter title |
Analysis of Intermediates of Steroid Transformations in Resting Cells by Thin-Layer Chromatography (TLC)
|
---|---|
Chapter number | 24 |
Book title |
Microbial Steroids
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Published in |
Methods in molecular biology, July 2017
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DOI | 10.1007/978-1-4939-7183-1_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7182-4, 978-1-4939-7183-1
|
Authors |
Govinda Guevara, Julián Perera, Juana-María Navarro-Llorens |
Abstract |
Thin-layer chromatography (TLC) is a useful and convenient method for the analysis of steroids due to: its simple sample preparation, low time-consuming process, high sensitivity, low equipment investment and capacity to work on many samples simultaneously. Here we describe a TLC easy protocol very useful to analyze steroid molecules derived from a biotransformation carried out in wild-type and mutant resting cells of Rhodococcus ruber strain Chol-4. Following this protocol, we were able to detect the presence or the absence of some well-known intermediates of cholesterol catabolism in Rhodococcus, namely AD, ADD, and 9OHAD. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 17% |
Professor > Associate Professor | 1 | 17% |
Student > Doctoral Student | 1 | 17% |
Student > Master | 1 | 17% |
Unknown | 2 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Environmental Science | 1 | 17% |
Biochemistry, Genetics and Molecular Biology | 1 | 17% |
Immunology and Microbiology | 1 | 17% |
Materials Science | 1 | 17% |
Unknown | 2 | 33% |