Chapter title |
Genome Sequencing of Steroid Producing Bacteria Using Ion Torrent Technology and a Reference Genome
|
---|---|
Chapter number | 4 |
Book title |
Methods in Molecular Biology
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7183-1_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7182-4, 978-1-4939-7183-1
|
Authors |
Sola-Landa, Alberto, Rodríguez-García, Antonio, Barreiro, Carlos, Pérez-Redondo, Rosario, Alberto Sola-Landa, Antonio Rodríguez-García, Carlos Barreiro, Rosario Pérez-Redondo |
Abstract |
The Next-Generation Sequencing technology has enormously eased the bacterial genome sequencing and several tens of thousands of genomes have been sequenced during the last 10 years. Most of the genome projects are published as draft version, however, for certain applications the complete genome sequence is required.In this chapter, we describe the strategy that allowed the complete genome sequencing of Mycobacterium neoaurum NRRL B-3805, an industrial strain exploited for steroid production, using Ion Torrent sequencing reads and the genome of a close strain as the reference. This protocol can be applied to analyze the genetic variations between closely related strains; for example, to elucidate the point mutations between a parental strain and a random mutagenesis-derived mutant. |
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