Chapter title |
Intra- and Extra-cellular Proteome Analyses of Steroid-Producer Mycobacteria
|
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Chapter number | 6 |
Book title |
Methods in Molecular Biology
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7183-1_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7182-4, 978-1-4939-7183-1
|
Authors |
Barreiro, Carlos, Morales, Alejandro, Vázquez-Iglesias, Inés, Sola-Landa, Alberto, Carlos Barreiro, Alejandro Morales, Inés Vázquez-Iglesias, Alberto Sola-Landa |
Abstract |
The importance of the pathogenic mycobacteria has mainly focused the omic analyses on different aspects of their clinical significance. In contrast, those industrially relevant mycobacteria have received less attention, even though the steroids market sales in 2011, in example, were estimated in $8 billion.The extra-cellular proteome, due to its relevance in the sterols processing and uptake; as well as the intra-cellular proteome, because of its role in steroids bioconversion, are the core of the present chapter. As a proof of concept, the obtaining methods for both sub-proteomes of Mycobacterium neoaurum NRRL B-3805, a relevant industrial strain involved in steroids production, have been developed. Thus, procedures and relevant key points of these proteomes analyses are fully described. |
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Professor > Associate Professor | 2 | 20% |
Researcher | 2 | 20% |
Unknown | 1 | 10% |
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Chemistry | 1 | 10% |
Unknown | 2 | 20% |