Chapter title |
Selection of Biodegrading Phytosterol Strains
|
---|---|
Chapter number | 9 |
Book title |
Methods in Molecular Biology
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7183-1_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7182-4, 978-1-4939-7183-1
|
Authors |
Mondaca, María-Angélica, Vidal, Maricel, Chamorro, Soledad, Vidal, Gladys, María-Angélica Mondaca, Maricel Vidal, Soledad Chamorro, Gladys Vidal |
Abstract |
The phytosterol-biotransforming strains were selected from Mycobacterium sp., using a high concentration of β-sitosterol. The selection was made by culturing the strains in a medium enriched with 14 g β-sitosterol/L as the unique source of carbon. During 2 months, the bacterial cultures were transferred successively. The extraction of the biotransformation products was made with methanol and ethyl acetate. The qualitative and quantitative analysis was made by means of thin-layer chromatography, gas-liquid chromatography (GLC), and GLC-mass spectrometry. Under these conditions, it was observed that after seven transfers, the strains Mycobacterium sp. MB-3683 and the Mycobacterium fortuitum B-11045 increased their biotransformation capacity from 20% to 64% and from 34% to 55%, respectively. The products in the highest proportion identified for each trial were androstenedione and androstadienedione. The results suggest that the high substrate concentration could be a selective mechanism to obtain strains more efficient in the biotransformation of β-sitosterol into steroidal bases. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 20% |
Professor > Associate Professor | 2 | 20% |
Researcher | 2 | 20% |
Professor | 1 | 10% |
Student > Doctoral Student | 1 | 10% |
Other | 1 | 10% |
Unknown | 1 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 40% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 10% |
Chemical Engineering | 1 | 10% |
Agricultural and Biological Sciences | 1 | 10% |
Economics, Econometrics and Finance | 1 | 10% |
Other | 0 | 0% |
Unknown | 2 | 20% |