Chapter title |
Scale-Up of Phytosterols Bioconversion into Androstenedione
|
---|---|
Chapter number | 14 |
Book title |
Methods in Molecular Biology
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7183-1_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7182-4, 978-1-4939-7183-1
|
Authors |
Martínez-Cámara, Sonia, Bahíllo, Esther, Barredo, José-Luis, Rodríguez-Sáiz, Marta, Sonia Martínez-Cámara, Esther Bahíllo, José-Luis Barredo, Marta Rodríguez-Sáiz |
Abstract |
Phytosterols, generated as a by-product of vegetable oils or wood pulp, contain the cyclopentane-perhydro-phenanthrene nucleus, and can be converted into steroid intermediates by removing the C17 side chain. This chapter shows the scale-up, from flask to fermentor, of the phytosterols bioconversion into 4-androstene-3,17-dione (androstenedione; AD) with Mycobacterium neoaurum B-3805. Due to the fact that phytosterols and AD are nearly insoluble in water, two-phase systems and the use of chemically modified cyclodextrins have been described as methods to solve it. Here we use a water-oil two-phase system that allows for the bioconversion of up to 20 g/L of phytosterols into AD in 20 L fermentor. |
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