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Microbial Steroids

Overview of attention for book
Cover of 'Microbial Steroids'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Steroid Bioconversions
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    Chapter 2 Chemical Pathways of Corticosteroids, Industrial Synthesis from Sapogenins
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    Chapter 3 Genome Sequencing of Steroid-Producing Bacteria with Illumina Technology
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    Chapter 4 Genome Sequencing of Steroid Producing Bacteria Using Ion Torrent Technology and a Reference Genome
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    Chapter 5 RNA-Seq-Based Comparative Transcriptomics: RNA Preparation and Bioinformatics
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    Chapter 6 Intra- and Extra-cellular Proteome Analyses of Steroid-Producer Mycobacteria
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    Chapter 7 Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain Mycobacterium neoaurum NRRL B-3805
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    Chapter 8 Identification and Characterization of the Genes and Enzymes Belonging to the Bile Acid Catabolic Pathway in Pseudomonas
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    Chapter 9 Selection of Biodegrading Phytosterol Strains
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    Chapter 10 Stigmasterol Removal by an Aerobic Treatment System
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    Chapter 11 Production and Biotransformation of Phytosterol Microdispersions to Produce 4-Androstene-3,17-Dione
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    Chapter 12 β-Sitosterol Bioconversion to Androstenedione in Microtiter Plates
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    Chapter 13 Bioconversion of Phytosterols into Androstenedione by Mycobacterium
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    Chapter 14 Scale-Up of Phytosterols Bioconversion into Androstenedione
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    Chapter 15 Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331
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    Chapter 16 Process for Biotransformation of Androsta-4-ene-3, 17-Dione (4-AD) to Androsta-1,4-Diene-3,17-Dione (ADD)
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    Chapter 17 Laboratory-Scale Hydroxylation of Steroids by P450BM3 Variants
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    Chapter 18 Obtaining of 11α-Hydroxyandrost-4-ene-3,17-dione from Natural Sterols
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    Chapter 19 Steroid 11-Alpha-Hydroxylation by the Fungi Aspergillus nidulans and Aspergillus ochraceus
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    Chapter 20 Biotransformation of DHEA into 7α,15α-diOH-DHEA
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    Chapter 21 Stabilization of Enzymes by Using Thermophiles
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    Chapter 22 Biocatalysis of Steroids with Mycobacterium sp. in Aqueous and Organic Media
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    Chapter 23 Downstream Process Synthesis for Microbial Steroids
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    Chapter 24 Analysis of Intermediates of Steroid Transformations in Resting Cells by Thin-Layer Chromatography (TLC)
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    Chapter 25 A Ligand-Binding Assay to Measure the Affinity and Specificity of Sterol-Binding Proteins In Vitro
Attention for Chapter 15: Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331
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Chapter title
Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331
Chapter number 15
Book title
Methods in Molecular Biology
Published in
Methods in molecular biology, July 2017
DOI 10.1007/978-1-4939-7183-1_15
Pubmed ID
28710631
Book ISBNs
978-1-4939-7182-4, 978-1-4939-7183-1
Authors

García-Fernández, Julia, Martínez, Igor, Fernández-Cabezón, Lorena, Felpeto-Santero, Carmen, García, José-Luis, Galán, Beatriz, Julia García-Fernández, Igor Martínez, Lorena Fernández-Cabezón, Carmen Felpeto-Santero, José-Luis García, Beatriz Galán

Abstract

The C19 steroid 1,4-androstadiene-3,17-dione (androstadienedione, ADD) is an added value product used as a synthon in the pharmaceutical industry for the commercial production of corticosteroids, mineralocorticoids, oral contraceptives, and other pharmaceutical steroids. Phytosterol biotransformation catalyzed by microbial whole cells is actually a very well-established research area in white biotechnology. The protocol below provides detailed information on ADD production by the mutant CECT 8331 of Mycobacterium smegmatis mc(2)155 using phytosterols as raw material in a lab scale. This protocol describes the bioconversion of phytosterols into ADD in a single fermentation step.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 2 29%
Researcher 2 29%
Student > Bachelor 1 14%
Professor 1 14%
Unknown 1 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 29%
Chemical Engineering 1 14%
Pharmacology, Toxicology and Pharmaceutical Science 1 14%
Chemistry 1 14%
Unknown 2 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 July 2017.
All research outputs
#15,418,980
of 22,988,380 outputs
Outputs from Methods in molecular biology
#5,333
of 13,150 outputs
Outputs of similar age
#195,563
of 312,216 outputs
Outputs of similar age from Methods in molecular biology
#98
of 274 outputs
Altmetric has tracked 22,988,380 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,150 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 312,216 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 274 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.

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