Chapter title |
Preparation and Characterization of Alginate–Chitosan Microcapsule for Hepatocyte Culture
|
---|---|
Chapter number | 15 |
Book title |
Cell Microencapsulation
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6364-5_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6362-1, 978-1-4939-6364-5
|
Authors |
Lanjuan Li, Yanhong Zhang, Xiaoping Pan |
Editors |
Emmanuel C. Opara |
Abstract |
This method is used to prepare microencapsulation of hepatocytes. The key steps in the protocol are (a) isolation of porcine hepatocytes, followed by (b) production of hepatocytes encapsulation, and (c) evaluation of encapsulations and hepatocytes. This method of collecting porcine hepatocytes was first used in 2003 (Maruyama et al. Cell Transplant 12:593-598, 2003). Cell encapsulation technology was first introduced by Chang (Science 146:524-525, 1964) and later by Lim and Sun in 1980 (Science 210:908-910, 1980). Since then, it has been well adopted in tissue engineering and controlled delivery of biological therapeutics such as bioartificial liver. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 1 | 11% |
Student > Bachelor | 1 | 11% |
Student > Ph. D. Student | 1 | 11% |
Student > Master | 1 | 11% |
Researcher | 1 | 11% |
Other | 0 | 0% |
Unknown | 4 | 44% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 22% |
Agricultural and Biological Sciences | 2 | 22% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 11% |
Unknown | 4 | 44% |