Chapter title |
In Vitro Biochemical Characterization of Cytokinesis Actin-Binding Proteins
|
---|---|
Chapter number | 12 |
Book title |
Yeast Cytokinesis
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3145-3_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3144-6, 978-1-4939-3145-3
|
Authors |
Dennis Zimmermann, Alisha N. Morganthaler, David R. Kovar, Cristian Suarez, Zimmermann, Dennis, Morganthaler, Alisha N., Kovar, David R., Suarez, Cristian |
Editors |
Alberto Sanchez-Diaz, Pilar Perez |
Abstract |
Characterizing the biochemical and biophysical properties of purified proteins is critical to understand the underlying molecular mechanisms that facilitate complicated cellular processes such as cytokinesis. Here we outline in vitro assays to investigate the effects of cytokinesis actin-binding proteins on actin filament dynamics and organization. We describe (1) multicolor single-molecule TIRF microscopy actin assembly assays, (2) "bulk" pyrene actin assembly/disassembly assays, and (3) "bulk" sedimentation actin filament binding and bundling assays. |
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Mendeley readers
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Unknown | 1 | 8% |