Chapter title |
Targeting Aflatoxin Biosynthetic Genes
|
---|---|
Chapter number | 10 |
Book title |
Mycotoxigenic Fungi
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6707-0_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6705-6, 978-1-4939-6707-0
|
Authors |
Ali Y. Srour, Ahmad M. Fakhoury, Robert L. Brown, Srour, Ali Y., Fakhoury, Ahmad M., Brown, Robert L. |
Abstract |
Chemical detoxification and physical destruction of aflatoxins in foods and feed commodities are mostly unattainable in a way that preserves the edibility of the food. Therefore, preventing mycotoxins in general and aflatoxins in particular from entering the food chain is a better approach. This requires early detection of the aflatoxin-causing organisms. Detection and quantification of aflatoxin-producing fungi has always been a challenge, especially within species of Aspergillus and Penicillium. Culture-based methods require a high level of expertise and a list of sophisticated equipment. Furthermore, even for a trained taxonomist, species that are identical in morphology, physiology, and nutritional aspects can be challenging to classify. Fungal taxonomy has changed over the past few decades; more species are being reclassified, and new species are being described due to advances in sequencing and genome assembly. These developments make the use of PCR-based approaches practical, rapid, and more reliable for the identification of fungi to the species level. This chapter presents a variety of protocols to detect and quantify aflatoxin-producing fungi using mycotoxin biosynthesis pathway genes. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 5 | 22% |
Student > Postgraduate | 2 | 9% |
Student > Ph. D. Student | 2 | 9% |
Student > Doctoral Student | 1 | 4% |
Student > Bachelor | 1 | 4% |
Other | 3 | 13% |
Unknown | 9 | 39% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 22% |
Agricultural and Biological Sciences | 5 | 22% |
Medicine and Dentistry | 2 | 9% |
Engineering | 1 | 4% |
Unknown | 10 | 43% |